DESCRIPTION
Source
HEK293 cells（with a C-terminal 6-His tag）
Predicted N-terminal ：Met
Lot#: 202005-02
Predicted Molecular Mass
27.1kDa
SPECIFICATIONS
SDS- PAGE
~27.1 kDa, reducing conditions.
Purity
≥ 95%（as determined by SDS-PAGE）
Concentration
1.0 mg/mL
Format
Clear Liquid
Formulation
Supplied as a 10 mM sterile PBS,ph7.4.
PREPARATION AND STORAGE
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Avoid repeated freeze-thaw cycles. Store at -20 to -80 ℃
BACKGROUND
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain
receptors on the host cell. Known receptors bind S1 are ACE2. The spike is essential for both host
specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping
of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein
of coronaviruses is known to be essential in the binding of the virus to the host cell at the
advent of the infection process. It's been reported that SARS-CoV-2 can infect the human
respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein
contains two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is
responsible for recognizing the cell surface receptor. The main functions for the Spike protein
are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts
and specificity of the virus; Main component to bind with the neutralizing antibody; Key target
for vaccine design; Can be transmitted between different hosts through gene recombination or
mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
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