DESCRIPTION
Source： Peptidyl Arginine Deiminase, from rabbit skeletal muscle
Description In
vitro, calcium is required for peptidylarginine deiminase activity.
Predicted Molecular Mass 80 kDa
SPECIFICATIONS
SDS- PAGE 80kDa, reducing conditions.
Purity ≥ 90%
Activity One unit will produce 1 μN-α moles of benzoylcitrulline ethyl ester per hour
from BAEE at 55°C, pH 7.2.
Presentation 20 mM Tris-HCl，pH 7.4， 10 mM 2-Mercaptoethanol、1 mM EDTA ，10% glycerol
Endotoxin Level No data available
PREPARATION AND STORAGE
Shipping : The product is shipped with dry ice or equivalent. Upon receipt, store it
immediately at the temperature recommended below.
Stability & Storage： 12 months from date of receipt, -20 to -80 °C as supplied.
3 months, 2 to 8 °C under sterile conditions
Application: Peptidylarginine deiminase is an enzyme that converts arginine to citrulline.
BACKGROUND
Peptidylarginine deiminase has been used in a study evaluating a promising novel biomarker for the early diagnosis of rheumatoid arthritis. Studies have also been conducted to investigate the autopathogenic association of periodontitis and rheumatoid arthritis.
References
1. Eva A V Moelants et
al.（2012）Citrullination
of TNF-α by peptidylarginine deiminases reduces its capacity to stimulate the
production of inflammatory chemokines.
2. Leendert A Trouw et al.（2012）Closing the serological gap: promising novel biomarkers for the early diagnosis of rheumatoid arthritis.
3. Jason S Knight et al. （2014）Peptidylarginine deiminase inhibition reduces vascular damage and modulates innate immune responses in murine models of atherosclerosis.
4. Reinout Raijmakers et al.（2007）Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro.
5. Maria A Christophorou et al.（2014）Citrullination regulates pluripotency and histone H1 binding to chromatin.
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