DESCRIPTION
Source Mouse
Clone Q1E2
Clonality Monoclonal Antibody
Applications ELISA, CLIA, FIA, etc.
SPECIFICATIONS
Product Name: Galectin-3
Purity >90%
Storage Buffer Lyophilized with PBS, pH 7.4,
Isotype IgG1
Concentration 2.1mg/mL（Brandford）
PREPARATION AND STORAGE
Shipping This product is shipped at 4℃
Stability & Storage Avoid repeated freeze-thaw cycles
This product is stable for 12 months from data of receipt when stored at -20 ℃to -80 ℃
ACTIVITY ASSAY PROTOCOL
Principles
Anti-Human Galectin-3 assay is a microtiter plate-based immunoassay for the quantitative determination of galectin-3 levels in human serum and plasma.
·Anti-Human Galectin-3 assay utilizes two monoclonal antibodies against galectin-3. The first monoclonal anti-human galectin-3 antibody is coated onto the surface of the wells in a microtiter plate and serves as the capture antibody to bind galectin-3 molecules in samples, while the other rabbit monoclonal anti-human galectin-3 antibody is provided in solution and functions as the tracer antibody for detecting galectin-3 molecules bound to the capture antibody.
· RMI provides ready-to-use microtiter plates and a monoclonal HRP-conjugated anti-galectin-3 antibody to support ELISA assay with procedures described below. Meanwhile, RMI offers other customized labeling on our tracer anti-galectin-3 for the purposes of FIA (Fluorescent Immunoassay) and CLIA (Chemiluminescent Immunoassays) that could be monitored at various wavelengths.
Test Procedure
1. Samples are introduced in duplicate into the wells and incubated. During this incubation, the galectin-3 present in the standards and specimens binds to the capture antibody coated onto the well surface. Subsequent washing removes all unbound material introduced with the samples, including unbound galectin-3.
2. The tracer antibody, a horseradish peroxidase (HRP)-labeled anti-galectin-3 antibody During this time, an antibody-antigen-antibody complex is formed.
3. After a wash step to remove any unbound tracer antibody, the tetramethylbenzidine (TMB) substrate is added,yielding a blue color in the presence of HRP.
The color development is stopped after 37℃,5 minutes by the addition of sulfuric acid, changing the color to yellow,
which is read at an absorbance of 450 nm.
4. The absorbance is proportional to the galectin-3 levels in the specimens.
[1] Meijers WC, van der
Velde AR, Pascual-Figal DA, et al. Galectin-3 and post-myocardial
infarction cardiac remodeling [J]. Eur J Pharmacol, 2015,
763(PtA): 115-121. DOI:
10.1016/j.ejphar.2015.06.025
[2] Lakomkin SV, Skvortsov AA, Goriunova TV, et al.
Galectin-3--a new biomarker for
diagnostics and outcome of chronic heart failure [J].
Kardiologiia, 2012, 52(3): 45-52
[3] Boer RA, Yu L, van Veldhuisen DJ. Galectin-3 in
cardiac remodeling and heart failure
[4] Sandwich_ELISA
[5]半乳糖凝集素3与急性冠脉综合征预后相关性的研究进展
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