DESCRIPTION Source: E. Coli(with a Cterminal 6His tag ) Predicted N-terminal : Met Lot#: 202005-01 Predicted Molecular Mass: 50 kDa SPECIFICATIONS SDS- PAGE: ~50 kDa, reducing conditions. Purity: ≥ 95%(as determined by SDS-PAGE) Concentration: 1.2 mg/mL Format: Clear Liquid Formulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 300 mM NaCl, 1mM EDTA, pH 8.0.
PREPARATION AND STORAGE
Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Avoid repeated freeze-thaw cycles. Store at -20 to -80 ℃
BACKGROUND Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helicalribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.