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购买数量：
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DESCRIPTION
Source： E. coliderived
Accession： P19731
N-terminal Sequence Analysis Ala2
Predicted Molecular Mass 20.9 kDa
SPECIFICATIONS
SDS- PAGE 20.9 kDa, reducing conditions.
Activity Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al.(2000) J.
Cell Sci. 113:2385. The ED50 for this effect is 3 10 µg/mL.
Purity ≥ 96% by SDS-PAGE visualized with Silver Staining and quantitative densitometry
by Coomassie® Blue Staining.
Formulation Supplied as a 0.2 μm filtered solution in PBS, pH 7.4，5%Glycerol
Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method.
PREPARATION AND STORAGE
Shipping : The product is shipped with dry ice or equivalent. Upon receipt, store it
immediately at the temperature recommended below.
Stability & Storage： 12 months from date of receipt, -20 to -80 °C as supplied.
3 months, 2 to 8 °C under sterile conditions
ACTIVITY ASSAY PROTOCOL DATA
Bioactivity and SDS-PAGE Bioactivity was measured by its ability to agglutinate human red blood cells. Hadari,
Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 3 10 µg/mL.Purity
was measured by SDS-PAGE
Sandwich ELISA: Dilution curves of recombinant human galectin 3 (XJ-Ag-01) in sandwich ELISA.using
11L24 as the capture antibody and 9K21 as the detection antibody. Signal decreased as
the recombinant human galectin 3(XJ-Ag-01) dilution increased.
BACKGROUND
lGalectin-3 (Gal-3) is also known as LGALS3, It is the only chimera member
of the Galectins family. It is formed by cross-linking a glycosyl with a
non-glycosyl ligand. Gal-3 includes 3 binding domains, NH2 terminal binding
domain, 1 sugar structure recognition domain and 1 A unique domain rich in
glycine and proline repeats. LGALS3 / Galectin-3 is expressed in the nucleus,
cytoplasm, mitochondrion, cell surface, and extracellular space. LGALS3 /
Galectin-3 has been shown to be involved in the following biological processes:
cell adhesion, cell activation and chemoattraction, cell growth and
differentiation, cellcycle, and apoptosis.Galectin 3 is involved in regulating cell growth, anti-apoptosis, mediating cell adhesion, and participating in biological functions such as blood vessel formation and inflammation. Studies have shown that Galectin-3 is a marker of myocardial fibrosis and is associated with the occurrence of heart failure and an increased risk of death. The content of galectin-3 in patients with ventricular dysfunction will increase, and the content is directly related to the severity of heart failure.
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