DESCRIPTION
Source: E. Coli(with a Cterminal 6His tag )
Predicted N-terminal : Met
Lot#: 202005-01
Predicted Molecular Mass: 50 kDa SPECIFICATIONS
SDS- PAGE: ~50 kDa, reducing conditions.
Purity: ≥ 95%(as determined by SDS-PAGE)
Concentration: 1.2 mg/mL
Format: Clear Liquid
Formulation: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 300 mM NaCl, 1mM EDTA, pH 8.0.
PREPARATION AND STORAGE
Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the
temperature recommended below.
Stability & Storage: Avoid repeated freeze-thaw cycles. Store at -20 to -80 ℃
BACKGROUND
Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA
chaperone activity that may be involved in template switch. Nucleocapsid protein
is a most abundant protein of coronavirus. N protein packages the positive strand
viral genome RNA into a helicalribonucleocapsid (RNP) and plays a fundamental
role during virion assembly through its interactions with the viral genome and
membrane protein M. Plays an important role in enhancing the efficiency of
subgenomic viral RNA transcription as well as viral replication. Because of the
conservation of N protein sequence and its strong immunogenicity, the N protein
of coronavirus is chosen as a diagnostic tool.